Table 1.

Drug effect severity scores for C. elegans. C. elegans were grown in the presence of the indicated concentrations of drug and scored for visible phenotypes.

Embryos	DMSO	5 μM	25 μM	50 μM	75 μM	100 μM	125 μM	150 μM
Sertraline	0	0	1	4	4	4	4	4
Paroxetine	0	0	1	3	4	4	4	4
Chlorpromazine	0	0	0	1	3	4	4	4
L1	DMSO	5 μM	25 μM	50 μM	75 μM	100 μM	125 μM	150 μM
Sertraline	0	0	1	4	4	4	4	4
Paroxetine	0	0	1	1	4	4	4	4
Chlorpromazine	0	0	0	0	2	2	4	4
L3/L4	DMSO	5 μM	25 μM	50 μM	75 μM	100 μM	125 μM	150 μM
Sertraline	0	0	0	2	2	3	4	4
Paroxetine	0	0	0	0	1	2	4	4
Chlorpromazine	0	0	0	0	0	1	1	4
Adults	DMSO	5 μM	25 μM	50 μM	75 μM	100 μM	125 μM	150 μM
Sertraline	0	0	1	3	4	4	4	4
Paroxetine	0	0	0	1	4	4	4	4
Chlorpromazine	0	0	0	0	4	4	4	4

All treatments were performed in duplicate wells, N = 50–100 worms/well, with each experiment repeated at least 3 times. Points were assigned for each scored effect based on the scoring descriptors below after 48 h of drug exposure and additive for each phenotype observed, with a maximum allowed total score of 4. Scoring descriptors were assigned when the entire test population exhibited the phenotype. Arrested = 1; Developmental delay = 1; Lethargic = 1; Paralyzed = 1; Toxic (>90% lethality) = 2; Dead = 4.