FIG 2.

Transcriptional analysis of cry64Ba and cry64Ca. (A) Shown here is a schematic diagram of distributions of cry64 genes and primers used for transcription analysis. (B) The electrophoresis pattern of the RT-PCR analysis for the cry64 operon is shown. Lane 1, fragments amplified by BaF/BaR; lane 2, fragments amplified by CaF/CaR; lane 3, fragments amplified by BaF/CaR, a PCR product that covered the gap between cry64Ba and cry64Ca. DNase-digested total RNA was used as the negative control while genomic DNA was used as the positive control. The cDNA was synthesized from total RNA and used for the transcriptional analysis.