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. 2018 Jan 17;84(3):e01996-17. doi: 10.1128/AEM.01996-17

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FIG 4 — Expression and purification of Cry64Ba and Cry64Ca in HD73⁻. (A) Physical maps of recombinant plasmids containing cry64Ba and cry64Ca clones. (B) SDS-PAGE analysis of Cry64Ba and Cry64Ca proteins expressed in HD73⁻ is shown. Lane 1, HD73⁻; lane 2, HD73⁻ harboring pHT1A-64Ba; lane 3, HD73⁻ harboring pHT1A-64Ca; lane 4, HD73⁻ harboring pHT1A-64Ba/Ca (target bands are marked with an arrow); lane 5, purified Cry64Ba and Cry64Ca proteins. (C) Gel filtration chromatography of purified Cry64Ba and Cry64Ca proteins is shown. BSA (66 kDa) was used as the molecular mass standard.