FIG 6.
Binding assay of fluorescein-labeled Cry64Ba-Cry64Ca to BBMV of L. striatellus. (A) Saturation binding assay of Cry64Ba/Cry64Ca to L. striatellus BBMV is shown. A fixed amount of BBMV proteins (10 μg) was used to bind Cry64Ba/Cry64Ca (0 to 900 nmol/liter). (B) Competition binding assay of Cry64Ba/Cry64Ca to L. striatellus BBMV is shown. The binding of 50 nmol/liter labeled Cry64Ba/Cry64Ca to L. striatellus BBMV was tested with increased concentrations of unlabeled Cry64Ba/Cry64Ca or Cry1Ac as a competitor.
