Characterization of FAE activities of recombinant R18 and TH2-18. (A) SDS-PAGE analysis of rR18 and rTH2-18. Lane 1, protein standard; lane 2, rR18; lane 3, rTH2-18. Proteins (10 μg) were loaded into the respective lanes. (B to D) Effects of temperature (B), pH (C), and thermostability (D) on FAE activities of rR18 and rTH2-18. Values represent the averages of data from three independent experiments. Error bars represent standard deviations.