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Construction of the sandwich-type chimeras and in vitro mutagenesis of rTH2-18. (A) Schematic illustration of the primary structures of four sandwich-type chimeras. The regions marked in black and white represent the sequences of R18 and TH2-18, respectively. (B) Amino acid sequences of chimeric positions in R18, TH2-18, and TH2-18PG. (C) CD spectra of rR18, rTH2-18, and rTH2-18PG.
