TABLE 3.
In vitro enzyme activities of ARD, ADH, XLR, and XYL2 of YlSR102 growing on single xylose, single arabinose, and mixed pentose sugars
| Substrate |
In vitro enzyme activitya (U) |
||||||
|---|---|---|---|---|---|---|---|
| ARD |
ADH |
XLR |
XYL2 (NAD+) | ||||
| NADH | NADPH | NAD+ | NADP+ | NADH | NADPH | ||
| Xyl | ND | ND | 0.07 ± 0.01 | ND | ND | 0.06 ± 0.02 | 2.48 ± 0.50 |
| Ara | ND | 0.75 ± 0.18 | 0.07 ± 0.01 | ND | ND | 0.35 ± 0.05 | 1.36 ± 0.22 |
| Xyl + Ara | ND | 1.62 ± 0.14 | 0.06 ± 0.00 | ND | ND | 0.59 ± 0.09 | 2.10 ± 0.53 |
a
NAD(P)+ and NAD(P)H were used as cofactors for enzyme assays. ND, not detected.