. 2018 Jan 17;84(3):e02146-17. doi: 10.1128/AEM.02146-17

TABLE 4.

Primers used in this study

Gene/protein/plasmid	Primer	Sequence
Primers used for constructing plasmids
Yli URA3	URA3_Fwd	CGGATATACTTGCTTGAATATACAGTAGTATGCGGCCGCTCGACGCCCAGAGAGCCATTG
Yli URA3	URA3_Rev	GTTTTTTGGGACACAAATACGCCGCCAACCCGGTCTCTCGAGAAACACAACAACATGC
pSR008	pSR008_Fwd	AGAGACCGGGTTGGCGGCGTATTTG
pSR008	pSR008_Rev	AGCGGCCGCATACTACTGTATATTCAAG
pSR001, pSR019–pSR039	pSR001_Fwd	TCATGTAATTAGTTATGTCACGCTTAC
pSR001, pSR019–pSR039	pSR001_Rev	TTTGAATGATTCTTATACTCAGAAG
trp1_Yli	TRP1_YL_Fwd	GTTAAGCATTTCCTTCTGAGTATAAGAATCATTCAAAATGTCCACTAGTGCTATGACC
trp1_Yli	TRP1_YL_Rev	GGGCGTGAATGTAAGCGTGACATAACTAATTACATGACTAAGAGGACTCGGAGAAGTC
trp2_Yli	TRP2_YL_Fwd	CGTTAAGCATTTCCTTCTGAGTATAAGAATCATTCAAAATGGCCATTATTGTGGCTG
trp2_Yli	TRP2_YL_Rev	GCGTGAATGTAAGCGTGACATAACTAATTACATGACTAATCCGAATCAAATCCAGAATCG
trp4_Yli	TRP4_YL_Fwd	AGCATTTCCTTCTGAGTATAAGAATCATTCAAAATGATTGGAAACGCTCAAATTAAC
trp4_Yli	TRP4_YL_Rev	GAGGGCGTGAATGTAAGCGTGACATAACTAATTACATGATTACAATTGAGAGGGAGGG
trp6_Yli	TRP6_YL_Fwd	CGTTAAGCATTTCCTTCTGAGTATAAGAATCATTCAAAATGGGCTTCAGAGGCC
trp6_Yli	TRP6_YL_Rev	GGCGTGAATGTAAGCGTGACATAACTAATTACATGATTAAACATGTCTGGTTTCCTCTTG
trp7_Yli	TRP7_YL_Fwd	CGTTAAGCATTTCCTTCTGAGTATAAGAATCATTCAAAATGGGACGAAACTGGCTAG
trp7_Yli	TRP7_YL_Rev	GAGGGCGTGAATGTAAGCGTGACATAACTAATTACATGATTAAGCTTGAGAAACGTTCTC
trp8_Yli	TRP8_YL_Fwd	CGTTAAGCATTTCCTTCTGAGTATAAGAATCATTCAAAATGTTTTCGTTAACGGGC
trp8_Yli	TRP8_YL_Rev	GAGGGCGTGAATGTAAGCGTGACATAACTAATTACATGATTATACCGGAGGTTGAGGG
trp11_Yli	TRP11_YL_Fwd	CGTTAAGCATTTCCTTCTGAGTATAAGAATCATTCAAAATGAAAGACTTCCTCGCC
trp11_Yli	TRP11_YL_Rev	GAGGGCGTGAATGTAAGCGTGACATAACTAATTACATGACTACGCTGTCTCGATTCGAAC
trp12_Yli	TRP12_YL_Fwd	GCATTTCCTTCTGAGTATAAGAATCATTCAAAATGATACTTTTTTGGTTACACAGAG
trp12_Yli	TRP12_YL_Rev	GAGGGCGTGAATGTAAGCGTGACATAACTAATTACATGATTATTGATGAGTGGTGGTGTC
trp13_Yli	TRP13_YL_Fwd	GTTAAGCATTTCCTTCTGAGTATAAGAATCATTCAAAATGAAGCTGTTTAAACGAGAAGC
trp13_Yli	TRP13_YL_Rev	GAGGGCGTGAATGTAAGCGTGACATAACTAATTACATGACTATCCACGAATAGTGGCAC
trp14_Yli	TRP14_YL_Fwd	TCGTTAAGCATTTCCTTCTGAGTATAAGAATCATTCAAAATGTCGCTGGACAAAAACC
trp14_Yli	TRP14_YL_Rev	GGGCGTGAATGTAAGCGTGACATAACTAATTACATGACTACTTCTTGTAGCCTCTCTTG
trp15_Yli	TRP15_YL_Fwd	CGTTAAGCATTTCCTTCTGAGTATAAGAATCATTCAAAATGTCGTCTATATCTTCGTCC
trp15_Yli	TRP15_YL_Rev	GAGGGCGTGAATGTAAGCGTGACATAACTAATTACATGACTACATGGTCCAAACCTCGG
trp16_Yli	TRP16_YL_Fwd	CGTTAAGCATTTCCTTCTGAGTATAAGAATCATTCAAAATGTCTTCCTATCCATCCGAG
trp16_Yli	TRP16_YL_Rev	GGGGAGGGCGTGAATGTAAGCGTGACATAACTAATTACATGATTAAGCAAGCTCCGCCG
trp17_Yli	TRP17_YL_Fwd	CGTTAAGCATTTCCTTCTGAGTATAAGAATCATTCAAAATGGTTTTTGGACGAGAAAAAG
trp17_Yli	TRP17_YL_Rev	GGGAGGGCGTGAATGTAAGCGTGACATAACTAATTACATGATTAAACGAACTCGGCAGTG
trp19_Yli	TRP19_YL_Fwd	TAAGCATTTCCTTCTGAGTATAAGAATCATTCAAAATGTTCTGGAAGAACATGAAAAATG
trp19_Yli	TRP19_YL_Rev	CGTGAATGTAAGCGTGACATAACTAATTACATGATTAACAATTCTCCACATGAATAACAC
trp21_Yli	TRP21_YL_Fwd	TAAGCATTTCCTTCTGAGTATAAGAATCATTCAAAATGTTCTGGAAAAACATGAAGAATG
trp21_Yli	TRP21_YL_Rev	GAGGGCGTGAATGTAAGCGTGACATAACTAATTACATGACTAACAGGTCTCCACGTGAAC
trp22_Yli	TRP22_YL_Fwd	CGTTAAGCATTTCCTTCTGAGTATAAGAATCATTCAAAATGTCGCACCGGCCCTG
trp22_Yli	TRP22_YL_Rev	GAGGGCGTGAATGTAAGCGTGACATAACTAATTACATGATCACCTATCAGCATTTTCACC
trp23_Yli	TRP23_YL_Fwd	ATCGTTAAGCATTTCCTTCTGAGTATAAGAATCATTCAAAATGTCAATCAAGTCGCTCTC
trp23_Yli	TRP23_YL_Rev	AGGGCGTGAATGTAAGCGTGACATAACTAATTACATGACTAGACACCATCTTTAGCAACC
Primers used in RT-PCR
L-ARD1	ARD1_rt Fwd	AGCTGCAGACCGACTACGTT
L-ARD1	ARD1_rt Rev	GCCTCCCAAGTCTCCTCGAC
L-ARD2	ARD2_rt Fwd	CGACACATTGACACCGCCTT
L-ARD2	ARD2_rt Rev	CGGTCGTGGTAAGTGACCCA
L-ARD3	ARD3_rt Fwd	AGCCCAAGACCTGGAAGCAA
L-ARD3	ARD3_rt Rev	CCTGGTTGACAGCAGGGACA
L-ARD4	ARD4_rt Fwd	GGGTGTCAAGCGAGAGGACA
L-ARD4	ARD4_rt Rev	GGCCAGTGGATGAGCAGCAT
L-ARD5	ARD5_rt Fwd	CGTCGACATGCTGCTGATCC
L-ARD5	ARD5_rt Rev	GCCTCCATCTGCTTCCAGGT
L-ARD6	ARD6_rt Fwd	CGCCATCAAGGACGGCTACA
L-ARD6	ARD6_rt Rev	ACAAACAGCTCCTCCCGCTT
L-ARD7	ARD7_rt Fwd	CCCATCGCTGTGGAAATCGC
L-ARD7	ARD7_rt Rev	CCGTTGGCTACAACACGTTCA
L-ARD8	ARD8_rt Fwd	TACTCCTTCCTGCCCAAGCG
L-ARD8	ARD8_rt Rev	CGTTGGACACGCCAATGGAC
L-ARD9	ARD9_rt Fwd	TCGCCTCTAGCTTCCAACGA
L-ARD9	ARD9_rt Rev	TGCCCTTCTGAGTAGCCCATC
L-ARD10	ARD10_rt Fwd	GGCAATGAAGCTGCCACTTGT
L-ARD10	ARD10_rt Rev	AGCTGCATGGTGCCCTCATA
L-ARD11	ARD11_rt Fwd	GGGCATCTCCGAATGGCTGA
L-ARD11	ARD11_rt Rev	CTTGGCCTTGGTCAGAGCCT
L-XLR1	XLR1_rt Fwd	AACGCCAAGCAGGTCGAGA
L-XLR1	XLR1_rt Rev	CCTTGTCCCACTCCTCGTTGT
L-XLR2	XLR2_rt Fwd	GGCTGAGCACCTCGCTAAGA
L-XLR2	XLR2_rt Rev	GCGTTGGCGACGAAGATGTC
L-XLR3	XLR3_rt Fwd	GCAGGACCTGGGTCTGGATG
L-XLR3	XLR3_rt Rev	CGTGCACAACTCGGTCGATG
L-XLR4	XLR4_rt Fwd	GTGGTGGTCTGGGTATCGCA
L-XLR4	XLR4_rt Rev	CGTTGGCGGTCTTGTACCAC
L-XLR5	XLR5_rt Fwd	TTAAGCGAATGCCCGGCAAC
L-XLR5	XLR5_rt Rev	CCGGCGTTAGCGATGACAAC
Primer used to confirm Y. lipolytica strains (P_TEF)	P_TEF_seq Fwd	GTGGTTGGGACTTTAGCCAAGGG