TABLE 2.
PCR primers used in this study
| Primers | Sequences (5′→3′)a | Source or reference |
|---|---|---|
| cheZUF KpnI | GGGGTACCTGCCCTTCAGCGTCTGC | This study |
| cheZUR NdeI | GGAATTCCATATGGCGTCCTGGATCGTCTC | This study |
| cheZDF AgeI | GACCGGTCGACCGACGAGAATGTGG | This study |
| cheZDR SacI | CGAGCTCGCTGCCGATCCTCTATGC | This study |
| cheZcomF KpnI | GGGGTACCGAAATCACGAGGCCGTAC | This study |
| cheZcomR BamHI | CGGGATCCCATCCATCAAGCCAGCAA | This study |
| SDMDF | TTCCAGGCCATCACCGGCCA | This study |
| SDMDR | TGGCCGGTGATGGCCTGGAA | This study |
| SDMQF | CATCACCGGCGCGCGCATC | This study |
| SDMQR | GATGCGCGCGCCGGTGATG | This study |
| RT-cheZ-F | AACCATCGACAATTCCATCG | This study |
| RT-cheZ-R | TGAAGACCATCAGCTTCACG | This study |
| RT-1833-F | GCGGTTGTCGAGATACCAGT | This study |
| RT-1833-R | ACCTCATCACCTTCGTGACC | This study |
| RT-1834-F | TAGATGAAGGGGCTGTCGAG | This study |
| RT-1834-R | GCGTGCTCTACAAGGTGGAC | This study |
| RT-3326-F | GGTTCCACGACGTGATCTTC | 40 |
| RT-3326-R | CGTCTCCTGAGTTTCCGAAC | 40 |
| RT-16S-F | ACGGATTTCTTCCAGCAATG | 40 |
| RT-16S-R | ACCGGCAGTCCCTTTAGAGT | 40 |
a
Engineered restriction sites are underlined.