FIG 1.

Growth of T. kivui in solid medium and natural competence for DNA uptake. (A) Sealed metal jar for anaerobic incubation of T. kivui at 65°C. (B) T. kivui embedded in agar medium formed yellow to brownish disc-like colonies. (C) Natural competence of T. kivui during growth on complex medium with glucose at 65°C. One-ml subsamples of two growing cultures were incubated with 250 ng of plasmid pMU131 (16), and then embedded in complex medium with or without 200 μg · ml⁻¹ kanamycin. Squares, representative growth curve; triangles, corresponding transformants per CFU.