FIG 3.
Physiology of the T. kivui ΔpyrE strain TKV002. (A) Growth of the wild type (DSM 2030, filled triangles), T. kivui TKV002 (open circles), and T. kivui TKV002 containing plasmid pKOM1 encoding pyrE under the control of Pkan (filled diamonds), without the addition of uracil. (B) Growth of T. kivui TKV002 with Pkan-pyrE reinserted into the genome in between open reading frames (ORFs) TKV_c24500 and TKV_c24520 (filled squares), and growth of T. kivui TKV002 in the presence of 40 μM uracil (closed circles). All experiments were performed in minimal medium with glucose (25 mM) at 65°C. Shown is one representative experiment out of three independent biological replicates.