FIG 5.
FruK is essential for fructose metabolism. (A) Growth of the T. kivui strain ΔfruK strain TKV003 (closed squares) and the wild-type strain DSM 2030 (open circles) in the presence of 40 μM uracil. Growth of T. kivui TKV003 with plasmid pKOM3 (with fruK under the control of Pkan) in the absence of uracil (closed triangles). All experiments were performed on complex medium with 25 mM fructose at 65°C. (B) Growth of T. kivui TKV003 (closed squares) in the presence of uracil and the wild-type strain DSM 2030 (open circles) on complex medium with 25 mM glucose. Shown are data from one representative experiment out of three independent biological replicates.