TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid name | Description | Source or reference |
|---|---|---|
| E. coli | ||
| JW543 | DH5α containing pSKW35 (apramycinR) | This study |
| JW544 | DH5α containing pSKW36 (apramycinR) | This study |
| C. bescii | ||
| JWCB52 | ΔpyrFA ldh::ISCbe4 Δcbe1::PS-layeracel0614(E1) (ura−/5-FOAR) | 6 |
| JWCB73 | JWCB52 containing pJGW07 (ura+/5-FOAS) | 18 |
| JWCB107 | JWCB52 containing pSKW35 (ura+/5-FOAS) | This study |
| JWCB108 | JWCB52 containing pSKW36 (ura+/5-FOAS) | This study |
| Plasmids | ||
| pJGW07 | E. coli/C. bescii shuttle vector containing the C. thermocellum pyrF gene (apramycinR) | 29 |
| pSKW12 | Intermediate vector (apramycinR) | 8 |
| pSKW35 | Expression vector for extracellular Tm_1848 (apramycinR) | This study |
| pSKW36 | Expression vector for intracellular Tm_1848 (apramycinR) | This study |