TABLE 2.
List of primers used in this study
| Primer name | Sequence (5′–3′)a | Restriction enzyme | Purpose |
|---|---|---|---|
| SK021 | CCGCCCGGGAAACGAACCAGCCCTAACCTCT | XmaI | To construct pSKW35 |
| SK055 | AGAGCATGCCATCACCATCACCATCACTAATAAT | SphI | |
| SK078 | CCGCCCGGGGTGCGATTCGGTTATTTTGATGACG | XmaI | To construct pSKW35 |
| SK079 | AGAGCATGCGCCCATCACAACTTCAACCCTG | SphI | |
| SK052 | TATAGGGCCCCTCACCAAACCTCCTTGTATGAT | ApaI | To construct pSKW36 |
| SK080 | TATAGGGCCCGTGCGATTCGGTTATTTTGATGACG | ApaI | |
| DC460 | AGAGAGCGATCGACAGTTTGATTACAGTTTAGTCAGAGCT | PvuI | To confirm transformants |
| DC228 | ATCATCCCCTTTTGCTGATG |
a
Italicized sequences indicate the recognition sites of the corresponding restriction enzymes.