TABLE 1.
Strains and plasmids
| Strain or plasmid | Relevant propertiesa | Reference or source |
|---|---|---|
| E. coli strains | ||
| DH5α | F− supE44 ΔlacU169 (ϕ80lacZΔM15) hsdR17(rK− mK+) recA1 endA1 gyrA96 thi-1 relA1 | TaKaRa Corp. |
| MT616 | pro-82 thi-1 hsdR17 supE44 recA56 (pRK600); Cmr | 18 |
| S. meliloti strains | ||
| 1021 | Wild type from SU47; Strr | Laboratory store |
| gor19 | Same as 1021 but with gor::pK19mob2ΩHMB; Strr, Neor | This study |
| cpgor19 | gor19 strain with pSRK19; Strr, Neor, Gmr | This study |
| Plasmids | ||
| pK19 | pK19mob2ΩHMB; Kmr | 17 |
| pK19gor | pK19 with 300-bp PCR-amplified fragment of gor; Kmr | This study |
| pSRK | Broad-host-range expression vector; Gmr | 19 |
| pSRK19 | pSRK with gor ORF; Gmr | This study |
a
Cmr, chloramphenicol resistant; Strr, streptomycin resistant; Neor, neomycin resistant; Gmr, gentamicin resistant; Kmr, kanamycin resistant.