Fig. 6. Relative transcript levels (RTL) from qRT-PCR analysis of copA transcription. Exponentially growing wild-type (WT), ΔcopY and copY mutant allelic cells were diluted into BHI and allowed to grow to approximately 0.2 OD₆₂₀. Cultures were then spiked with (grey) or without (black) 200 μM Cu and incubated for 1 h before harvesting cells and RNA extraction. The average and s.d. of three biological replicates are shown.