Fig. 7. 1H–15N HSQC spectra of intact wild-type (WT) (A) and C52A/C101A (B) Spn CopY in the apo-state (blue contours) and the Zn1 allosteric state (red contours). Spectra were acquired at 30 °C in 20 mM HEPES, 0.2 M NaCl, 5 mM TCEP, pH 6.0. Additional NMR spectra of Cu-bound CopY and the C-terminal regulatory domain fragment (68-131) are shown in Fig. S5.† .
