Table 3.
Measurements of binding activity for each immobilized Ab.
| Antibody for CEA | XL (RU) | XA (RU) | pI/MWL* | Activity (%) | # of ligand on surface x (108/mm2) |
|---|---|---|---|---|---|
| B5 sdAb | 1200 | 525 | 6.35/14.7 | 3.6 | 480 |
| B5-GS3K | 805 | 910 | 7.84/16.0 | 14.3 | 322 |
| B5-Rhiz | 1363 | 839 | 5.27/29.4 | 5.0 | 272 |
| CEA mAb | 4389 | 621 | 7.0/155 | 5.4 | 351 |
MWL (kDa) represents the molecular weight of the ligand (antibody). The molecular weight for CEA used to calculate the activity was 180 kDa.