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. 2018 Jan 17;8:875. doi: 10.1038/s41598-018-19246-9

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The diploid sac7Δ/Δ mutant is defective in the white-to-opaque switching. (a) Diploid cells of WUM5A (WT), sac7Δ/Δ (YSL504), sac7Δ/Δ + SAC7 (YSL532), and sac7Δ/Δ + WOR1^OE (YSL505) were spread onto Lee’s GlcNAc plates (pH 6.0, +PB) and incubated at 25 °C in the dark for 6 days before photography. (b) Cells from randomly selected red and white colonies as indicated were suspended in water and examined under a microscope. (c) The white-to-opaque switching frequency of the strains grown on Lee’s GlcNAc plates (pH 6.0, +PB). White colonies, red colonies and white colonies with red regions from each strain were counted respectively, and the switching frequency was calculated as the percentage of red colonies plus white colonies with red regions over total colonies. *p < 0.05; **p < 0.01 (Student’s t-test).