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. 2018 Jan 17;8:875. doi: 10.1038/s41598-018-19246-9

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The white-to-opaque switching defect of the sac7Δ/Δ mutant was further exacerbated by ras1Δ/Δ. (a) Cells of ras1Δ/Δ (YSL639), sac7Δ/Δ (YSL503), and sac7Δ/Δ ras1Δ/Δ (YSL623) were grown on Lee’s GlcNAc plates (pH 6.0, +PB) at 25 °C in the dark for 6 days before photography. (b) Representative images of white and opaque cells of the indicated strains. (c) The white-to-opaque switching frequency of the tested strains. *p < 0.05 (Student’s t-test).