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. 2018 Jan 17;8:968. doi: 10.1038/s41598-018-19213-4

Figure 5.

Figure 5

Effects of erastin on the activity of cystine uptake in xCT-deficient MEF expressing xCT with different Cys to Ser substitutions. xCT-deficient MEF were cultured for 24 h and then plasmids containing either wild-type xCT or the different Cys to Ser mutants in pEF-BOS were transfected into cells. After 48 h incubation, the uptake of 0.05 mM L-[14C]cystine was measured in the absence (filled bar) or presence (open bar) of 10 µM erastin. Bars represent the mean ± S.D. (n = 8 for wild-type xCT (mxCT); n = 7 for other mutant plasmids). P values were obtained by unpaired Student’s t test. Each small letters indicate statistical significance between mxCT and C86S in the absence of erastin, between the absence and presence of erastin in each cells. P values are a = 5 × 10−4, b = 2 × 10−4, c = 2 × 105, d = 1 × 10−3, e = 4 × 10−4, f = 1 × 10−8, g = 6 × 10−6, h = 8 × 10−6.