Figure 6.
Effect of a short time pre-exposure of erastin on the activity of cystine uptake and intracellular total glutathione in human ovary cell lines. A2780 and A2780DDP cells were seeded on 35 mm dishes (2 × 105 cells/dish), cultured for 24 h and exposed to 10 µM or 100 µM erastin for 5 min. After removing erastin from the medium, the cells were washed with 1 mL of fresh medium once and were allowed to grow in fresh medium in the presence or absence of 10 µM cisplatin. After culturing the cells for 24 h, the uptake of 0.05 mM L-[14C]cystine (A) and intracellular glutathione levels (B) were measured. Each point represents the mean ± S.D. (n = 4). P values were obtained by unpaired Student’s t test. Each small letters indicate statistical significance. P values are a = 6 × 10−3, b = 3.6 × 10−2, c = 1 × 10−4, d = 6 × 10−3, e = 2 × 10−3, f = 1 × 10−3, g = 3 × 10−4.