Table 1.

Primer sets using for site-directed mutagenesis.

C86S	forward	5′-TCTGCCTCTGAAGTACTGTCACTTTTT-3′
	reverse	5′-TACTCCAGAGGCAGACCAGAAAACCAG-3′
C158S	forward	5′-ATTCAATCTGAAATTCCTGAACTTGCA-3′
	reverse	5′-AATTTCAGATTGAATAAAAAATGGTTCC-3′
C197S	forward	5′-ACCTTTTCCAAGCTCACAGCAATTCTG-3′
	reverse	5′-GAGCTTGGAAAAGGTTAGGAAAATCTG-3′
C271S	forward	5′-GCAATCTCCATCTCCATGGCTATCATC-3′
	reverse	5′-GGAGATGGAGATTGCAAGGGGGATGGT-3′
C327S	forward	5′-CTCTCCTCCTTCGGCTCCATGAACGGT-3′
	reverse	5′-GCCGAAGGAGGAGAGGGCAACAAAGAT-3′
C435S	forward	5′-TTCACCTCCCTCTTCATGGTCGTCCTC-3′
	reverse	5′-GAAGAGGGAGGTGAAGGAAAATAGTGC-3′