Fig. 3.

Evaluation of MGBA’s sensitivity and specificity. a Serial dilutions of lectins and GBPs were used to assess the lower limit of detection of MGBA. Lowest limit of detection values were 1.76pMol (GSL-II), 0.06pMol (VVL and AAL) and 13.3pMol (E-Selectin). b Specificity for anti-glycan antibodies. The data for three representative antibodies are shown here and the data for other antibodies are shown in Supplementary Fig. 6. The glycans with similar structures without binding are also presented in the figure to show specificity of the binding. Mean of two replicates is presented and error bars are standard deviations of the two replicates. Each experiment was repeated three times