Figure 1.

CRISRP-Cas9 activity reported with the T7E1 Assay and Next-Generation Sequencing. (A) NHEJ frequency with the T7E1 assay. Representative gel images of T7E1-treated PCR products amplified from the target sites of GFP-negative controls (−) and edited pools (+). (B) NHEJ events in CRISPR-Cas9 targets reported by NGS (black bars) or the T7E1 assay (grey bars). Data represent the mean of three biological replicates ±SEM. (C) Indel size spectrum (x-axis) and frequency (y-axis) identified by targeted NGS. The top four most prevalent reads are shown below the sgRNA sequence (5′ to 3′) with corresponding deletions (black dashes) and insertions (red letters). Red arrows identify sgRNA cut sites. Data represent the mean of three biological replicates ±SEM.