Table 1.
Summary of sgRNA Targets.
| Target | Sequence (5′ → 3′) | GC (%) | Location |
|---|---|---|---|
| H1a | TCATATAGTCGCTTTTCTTNGG | 35 | Coding |
| H2 | AATGGGGACGATTGGGCAAANGG | 57 | Coding |
| H3 | CTCACCAGTACTCTGCTTTCNGG | 50 | Noncoding |
| H4 | TGTCTGGGGACACGTCTCCANGG | 57 | Coding |
| H5 | TCCTCAGCATCTTATCCGAGNGG | 52 | Coding |
| H6 | GAATGAAAATGCGGTTCTTGNGG | 48 | Coding |
| H7 | GTCATCTCTACCTGCGACCANGG | 57 | Coding |
| H8 | GTCCCCTTCTGCCCAATGGTNGG | 65 | Coding |
| H9 | CCGTCACTGAGACAGTGCGCNGG | 65 | Coding |
| M1b | TATAGCCAGGCGAGTCCCCANGG | 65 | Coding |
| M2 | GAGCATAGGCTATGACACAANGG | 48 | Noncoding |
| M3 | GAGGAAGGACGCCCCCAGCANGG | 70 | Noncoding |
| M4 | TGGAGACTGTGAAGGTGCTCNGG | 57 | Coding |
| M5 | AGGACTTCCCCGACACCCAGNGG | 35 | Coding |
| M6 | ACACTTTATTGTGCTTGTATNGG | 35 | Splice junction |
| M7 | AACCTCCTCGAACGCGGGAGNGG | 70 | Coding |
| M8 | TAGCCAAGTGCTACCGCGTANGG | 57 | Noncoding |
| M9 | CCGGGAATACGACGTGGGCNGG | 74 | Coding |
| M10 | GGCCTTGGCGTCCTGGTCTTNGG | 70 | Coding |
aHuman targets are denoted by H1–H9. bMouse targets are denoted by M1–M10. Sequence, %GC content, and annotated feature (Human GRCh38/hg38 Gencode v24 for human and GRCm38/mm10 GENCODE M14 for mouse).