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. 2018 Jan 17;8:992. doi: 10.1038/s41598-018-19368-0

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Flow cytometry analysis histograms of G0/G1-phase arrest in 24 h M22 treated A549 cells. (A) The G0/G1 peak increases with corresponding decrease in S and G2 peak in dose dependent manner. M22 treated cells at 13 μM showing an increased sub G1 peak indicating apoptosis. (B) The percent of cells in different phases of cell cycle are represented by bar diagram. Data are from 3 independent experiments and analyzed by one-way analysis of variance, ***p < 0.001 compared to control. PI fluorescence was measured using a flow cytometer with FL-2 filter.