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. 2018 Jan 17;8:1006. doi: 10.1038/s41598-018-19630-5

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Differences in biotin abundances in flight muscle of Manduca sexta across Diel Time and Age. (a) Female, (b) Male. The x-axis represents age post-eclosion at Day (D) and Night (N). The y-axis box plots indicate the scaled intensity mean (+) and median (−) values: upper and lower ranges of boxes indicate upper and lower quartiles, respectively; upper and lower whiskers indicate the maximum and minimum distributions of the data; small circles represent outlying data points. The adjusted P values for the significant comparisons are presented in Tables S1 and S2.