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. 2018 Jan 17;8:883. doi: 10.1038/s41598-018-19225-0

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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TBK1 is required for hMPV-mediated induction of lfTSLP. (a) WI-38 cells were transfected with siRNAs against TBK1 or control siRNA (siControl) prior to infection with hMPV for 18 hours. lfTSLP mRNA expression was assessed by qRT-PCR. (b) Immortalized TBK1 +/+ or TBK1 −/− MEF cells were infected with hMPV for 24 hours. Mouse TSLP (mTSLP) mRNA expression was assessed by qRT-PCR. Data are representative for at least 3 independent experiments. Error bars represent SD of 3 technical replicates. **p < 0.01, ***p < 0.001.