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. 2018 Jan 17;8:980. doi: 10.1038/s41598-018-19465-0

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Distribution of GFP-tagged Gal-13 in HeLa and 293 T cells. (A) Following transfection, GFP-tagged Gal-13 was found to be distributed in the cytoplasm and nucleus. This protein was mainly localized within the nucleus of HeLa cells. There are 4–6 dark areas in the nucleus. (B) The nucleus of HeLa cells was stained by Hoechst. (C) Merge of (A) and (B). Similar experiments were performed using 293 T cells. (D) The GFP-tagged Gal-13 in 293 T cells is visualized. (E) The 293 T cell nuclei were stained using Hoechst. (F) Merge of (D) and (E).