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. 2018 Jan 17;8:940. doi: 10.1038/s41598-017-18378-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Phylogenetic tree and the evolutionary scenario of the plastid gene losses in Chlorellales. (a) Maximum Likelihood (ML) tree constructed using 38 plastid-encoded proteins. Bootstrap support (BP) is indicated above the lines, and Bayesian posterior probability (BPP) is indicated below the lines. BP <50 or BPP <0.95 are not shown. Bold lines represent 100% BP and 1.00 BPP. The dotted branches are shown in half-length. (b) ML tree of 58 nucleus-encoded proteins. All the nodes were supported with 100% BP. (c) Evolutionary scenario of gene losses in P. wickerhamii, P. cutis, P. stagnora, and Helicosporidium sp. Eliminated plastid genes are indicated on the tree. Genes shown in green and blue have independently disappeared two and three times in the AHP lineage. Loss of group-I intron is presented by intron [trnL(UAA)].