Table 1.

Optimization of the concentration of magnetic beads and HE4-Ab-ALP.

Magnetic beads concentration (mg/mL)	HE4 level (pmol/L)	HE4-Ab-ALP (ug/mL)
		0.05	0.1	0.2
0.05	0	784	1670	2852
	50	19,631	32,239	50,163
	1000	726,893	1,561,379	1,778,456
0.1	0	1236	2365	4174
	50	38,926	53219	68,362
	10,000	1,182,536	1,957,056	2,170,327
0.2	0	3125	4721	7893
	50	55,224	83219	101120
	1000	2,380,375	2,531,973	3,039,672
0.3	0	5184	7420	9405
	50	71,027	87,932	133,032
	1000	2,642,198	3,238,745	3,125,371