Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Jan 17;8:902. doi: 10.1038/s41598-017-18832-7

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

PPM1A inhibits the inflammatory response of differentiated macrophages. (A) Relative baseline expression of GFP in THP-TNFα^Prom cells compared to THP-PPM1A-TNFα^Prom reporter cells was measured by flow cytometry. MFI: mean fluorescence intensity of GFP signal. (B,C) THP-1 and THP-PPM1A TNFα promoter reporter monocytes were differentiated with (B) PMA (100 ng/ml) or stimulated with (C) LPS (100 ng/ml), and at the indicated days post treatment, TNFα promoter activity was measured by flow cytometry as a function of the GFP signal (MFI). (D–G) PMA-differentiated THP-1 and THP-PPM1A macrophages were mock treated or stimulated with LPS (100 ng/ml) to induce the production of cytokines/chemokines. Production of (D) TNFα, (E) IL-1β, (F) IP-10, (G) MIP-1β into the culture supernatant were measured using antibody based bead multiplex assays. (H) Levels of TNFα production in unstimulated PMA-Differentiated THP-1, THP-PPM1A and THP-ΔPPM1A macrophages were measured using antibody based bead assay. Data in this figure represent the means ± S.D. of three independent experiments. *p < 0.01 relative to THP-1 control cells.