Fig. 3.
Poor presentation of nanoparticle formulated αGalCer by B cells. B220 + B cells were purified from mouse spleen by cell sorting. 200,000 B cells were mixed with different concentration of nanoparticle formulated αGalCer, and incubated with 100,000 NKT cells (DN32.D3) for 24 h.NKT cell activation was represented by their secretion of cytokine (IL2). CD11c + cells and CD11b + cells were studied in parallel. Data were representative of two independent experiments: (A) presentation of nanoparticle formulated αGalCer and (B) presentation of free αGalCer.