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The level of miR-186-5p was downregulated in HG-treated AC16 cells. AC16 cells were treated with HG (33 mM) for 48 h. The level of miR-186-5p in AC16 cells was measured via reverse transcription-quantitative polymerase chain reaction. Data are presented as the mean ± standard error of the mean (n=3). *P<0.05, **P<0.01 vs. control group. miR, microRNA; HG, high glucose; snRNA, small nuclear RNA.
