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. 2018 Jan 17;8:879. doi: 10.1038/s41598-018-19197-1

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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ILO-PC induces more prolonged activation of Rac1 and Rap1 GTPase signaling in pulmonary EC. EC monolayers were stimulated with ILO (0.5 µM) or ILO-PC (0.5 µM) for indicated periods of time. (A) Rac-GTP pulldown assay. (A) Rap-GTP pulldown assay. (C) Phosphorylation of Rac1 targets PAK1 and cortactin was evaluated by western blot with corresponding phospho-specific antibodies. Membrane probing for β-tubulin was used as a normalization control. Bar graphs depict quantitative densitometry analysis of western blot data; n = 3; *p < 0.05. Data are expressed as mean ± SD. Biological replicates indicated by (n). Statistical significance by one-way analysis of variance (ANOVA) and Tukey’s post hoc multiple-comparison test.