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. 2018 Jan 17;8:884. doi: 10.1038/s41598-018-19149-9

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Plasmodial RNAs are contained inside the EVs. (a) The RNAs are protected from proteinase K digestion, EVs were treated with proteinase K (5 mg/ml, close symbols) or untreated (open symbol) at 55 °C. At times indicated aliquots were removed and the RNA was isolated and the expression of (a) MAL5_18S, (b) 28 S rRNAs, (c) PFA0110w, was analyzed by qPCR. The mean + /− s.d. of one representative experiments of three is shown. (d) Elution profile of conditioned media collected from iRBC culture as measured by absorbance at 280 nm. Points represent the mean +/− s.d. of three experiments performed in triplicate. (e) Fractions from conditioned media were assayed for (e) PFA0110w, (f) MAL5_18S, (g) 28 S rRNA, (h) PF11_0381a and (i) miR451 using absolute quantification by qPCR. The mean + /− s.d. of one representative experiment is shown (n = 3).