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. 2018 Jan 17;8:978. doi: 10.1038/s41598-018-19379-x

Figure 4.

Figure 4

(a) An unprocessed AFM image displaying translocase complexes in lipid bilayer, supported on mica41, with lateral pixel resolution 3.9 nm. (b) All non-overlapping Hessian blobs, extracted from the raw image. Only bright blobs considered. Subpixel boundaries calculated to 1/3 pixel resolution (1.3 nm). (c) All Hessian blobs with a scale of (8 nm)2 (4 pixel units2) or greater, set manually to eliminate most few-pixel blobs. (d) All Hessian blobs with blob strength 4.9 · 10−19 or greater, set manually to capture visually prominent molecules.