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. 2018 Jan 17;8:978. doi: 10.1038/s41598-018-19379-x

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(a) The first image in the SecYEG data set, displaying individual translocons in lipid bilayer supported on a glass substrate¹¹. Candidate SecYEG molecules are manually labelled with circles of radius 14 nm, regardless of true particle size. (b) Particles identified by the Hessian blob algorithm, with the minimum blob strength set such that all candidate particles were identified while minimizing false positive detections. (c) Watershed algorithm, with the minimum pixel parameter set in the same manner. (d) Height threshold particles, with the height threshold parameter set in the same manner.