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. 2018 Jan 17;8:895. doi: 10.1038/s41598-018-19166-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Microscopy of OCT-MSNs and control particles contrast the results of drug-templating and diffusion gradient drug loading. SEM and TEM micrographs of drug-templated MSNs before drug release (a and b, respectively) show well defined and separated particles approximately 424 nm in diameter. Post-drug-release OCT-MSNs (SEM in c, TEM in d) show very clear surface pore openings and well resolved pores throughout the particle. Control OCT-loaded MCM-41 (SEM in e, TEM in f) exhibits fusing of particles and a layer of external drug (highlighted with arrows).