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. 2018 Jan 17;8:957. doi: 10.1038/s41598-017-19104-0

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effects of androgens on physical activity in C57BL/6J male mice. Time course of daily wheel running activity over a period of 19 days (A) and average distance run per day after two weeks of training (B). Note that the color of the stars in A represents the specific comparisons. Average distance run per day after two weeks of training during exposure to vehicle and treatment with increasing doses of enzalutamide (C). Ambulatory movement beam breaks over 23 h (D) and total cumulative ambulatory activity during the night (E). (F) Seminal vesicle and LA/BC weight following ORX and replacement with T or DHT. Data in panels A and C were analyzed using two-way repeated measures ANOVA and those in panels B and E–F were analyzed by one-way ANOVA. Data are presented as mean ± SEM. In (A,B) and (F), n = 5–10 animals per group; in (C), n = 6 animals per group; in (D,E), n = 10–11 animals per group. Statistical significance levels: ^{*, **, ***, ****}P < 0.05, 0.01, 0.001 and 0.0001. ENZA enzalutamide, LA/BC levator ani/bulbocavernosus, SV seminal vesicles.