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. 2018 Jan 17;8:957. doi: 10.1038/s41598-017-19104-0

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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SHBG inhibits the stimulatory effects of T on wheel running activity. Time course of daily wheel running activity over a period of 19 days (A), average distance run per day after two weeks of training (B), seminal vesicle and LA/BC weight (C) and concentration of E2 in brain homogenates (D) in SHBG-Tg and WT mice, both treated with T following ORX. Data in panel A were analyzed using two-way repeated measures ANOVA and those in panels B,D were analyzed by Student t-test. All data are mean ± SEM, 6 animals per group. Statistical significance levels: ^{*, **, ***, ****}P < 0.05, 0.01, 0.001 and 0.0001. LA/BC levator ani/bulbocavernosus, SV seminal vesicles.