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. 2018 Jan 17;8:977. doi: 10.1038/s41598-017-16667-w

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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C1-INH oligomers in plasma. Non-denaturing PAGE and immuno-blot analysis of plasma from patient T.M. (378 R/C) at different functional values of C1-INH. Equal plasma volumes were loaded in each line. Heat-treated plasma C1-INH (oligomer), normal human plasma (NHP) and the plasma of a patient known to have circulating oligomers (T167N)²³ are shown as reference. The primary Ab used was the 12-27-15 monoclonal antibody, revealed with a secondary Ab designed for chemiluminescent detection.