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. 2018 Jan 17;8:936. doi: 10.1038/s41598-018-19335-9

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Reaction pathway and HPLC-MS/MS analysis of cyclo-Pro-AAs products. (a) The reaction pathway of cyclo-Pro-AAs, AAs stands for amino acids; (b) HPLC profile for the reaction product cyclo-Pro-Met of Pro with Met and P₃m in aqueous solution; (c) MS profile for the product cyclo-Pro-Met (M = 228); (d) MS² profile for the product cyclo-Pro-Met. HPLC-MS/MS profile for the reaction products of Pro with other amino acid could be found in the Supplementary Fig. S5.