Skip to main content
. 2018 Jan 17;8:964. doi: 10.1038/s41598-018-19301-5

Figure 5.

Figure 5

P2RX4 controls the spinal BDNF pathway under long lasting peripheral inflammatory conditions. Immunostaining analyses of the phosphorylation of the BDNF pathway proteins, Erk1/2 (A) and the GluN1 subunit of the NMDA receptor (B), in inflammatory conditions. Increased immunostaining of both phospho-Erk1/2 and phospho-GluN1 is observed in the dorsal horn, ipsilateral of the CFA injection in P2RX4+/+ mice, but it is absent in P2RX4−/− mice. Note that, in P2RX4-deficient mice, phosphorylation of Erk1/2 is maintained in deep lamina. Scale bar 200 µm. (C) Representative cropped western blot of KCC2 expression in the dorsal horn of the spinal cord in control and inflammatory condition, ipsilateral of the CFA injection. (D) Quantification of KCC2 expression observed in (C). KCC2 is down regulated 24 h after CFA injection in the dorsal horn of WT mice, but not in P2RX4−/− mice. N = 3 independent experiments, n = 2 mice per condition. One Way ANOVA, *p < 0.05.