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. 2017 Nov 9;15(1):741–746. doi: 10.3892/ol.2017.7376

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Copyright: © Rui et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 2. — CTLA-4 shRNA knocked down CTLA-4 expression in CIK cells. (A) Agarose gel images and qPCR analysis indicated that CTLA-4 mRNA expression was down-regulated in shCTLA-4 lentiviral particles transduced CIK cells compared with mock transduced CIK cells or shControl lentiviral particles transduced CIK cells. *P<0.01. CTLA-4 mRNA expression levels were normalized to GAPDH. CTLA-4 mRNA expression from shControl lentiviral particles transduced CIK cells and shCTLA-4 lentiviral particle-transduced CIK cells were normalized to 1 for mock transduced CIK cells. (B) Western blot analysis indicated that CTLA-4 protein expression was down-regulated in shCTLA-4 lentiviral particles transduced CIK cells compared with mock transduced CIK cells or shControl lentiviral particles transduced CIK cells. *P<0.01. CTLA-4 protein expression levels were normalized to GAPDH. CTLA-4 protein expression from shControl lentiviral particles transduced CIK cells and shCTLA-4 lentiviral particles transduced CIK cells were normalized to 1 for mock transduced CIK cells. The data presented are mean values from three independent experiments and plotted as the mean ± standard error of the mean. CTLA-4, cytotoxic T lymphocyte-associated antigen 4; CIK, cytokine-induced killer; PMBC, peripheral blood mononuclear cells; qPCR, quantitative polymerase chain reaction; sh, short hairpin.