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. 2017 Nov 9;15(1):963–971. doi: 10.3892/ol.2017.7378

Figure 2.

Figure 2.

Expression of miR-1 and PIK3CA in transfected cells. TE-1 cells were transiently transfected with miR-1 mimics (50 nM) or the negative control. The cells were obtained after 48 h for analysis. (A) TaqMan RT-qPCR detection of miR-1 expression levels in TE-1 cells. (B) The level of PIK3CA mRNA expression was detected by RT-qPCR in TE-1 cells. (C) Cell lysates were prepared and used for western blotting with antibodies specific for PIK3CA, total Akt, p-Akt and survivin. Data are presented as the mean ± standard error from three independent experiments. **P<0.01 vs. the negative control. NC, negative control; RT-qPCR, reverse transcription-quantitative polymerase chain reaction; miR, microRNA; PIK3CA, phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit α; Akt, protein kinase B; p, phosphorylated.