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. Author manuscript; available in PMC: 2018 Jul 26.
Published in final edited form as: Methods Enzymol. 2017 Jul 26;597:359–376. doi: 10.1016/bs.mie.2017.06.030

Fig. 1.

Fig. 1

(A) Incubation of Qβ with Tn-NHS form Qβ-Tn conjugate. (B) FPLC chromatogram of Qβ-Tn. (C) TEM image of Qβ-Tn showing the capsid is intact after functionalization. The scale bar is 30nm. (D) LCMS QTOF ESI mass spectroscopy with data processing by Maximum Entropy deconvolution algorithm (MaxEnt™ 1) (Da Ren, Raimville, Wheat, Russell, & Mazzeo, 2004). Based on the MS data, the number of Tn is estimated to be 376 per capsid (Table 1).