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a) SDS-PAGE gel of expression of Vluc from HEK293T mammalian cells. Lanes 1: Precision Plus Protein™ Dual Color Standard and Lane 2 the expressed Vluc. The band was calculated to have approximately a molecular weight 66 kDa. b) The bioluminescence activity of the matched concentration of full-length Vluc from HEK293T compared with the purified tVluc expressed from SHuffle® Express E. coli.
