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. Author manuscript; available in PMC: 2018 Jan 18.
Published in final edited form as: Mol Immunol. 2016 Nov 26;81:67–75. doi: 10.1016/j.molimm.2016.11.020

Figure 3. Up-regulation of BCL6 by glucose deprivation is insensitive to IL-2-mediated inhibition of Bcl6.

Figure 3

Naïve CD4+ T cells isolated from C57BL/6 or IL-2 KO mice were cultured under complete (+GLU) or glucose (GLU-) deprivation media for 72 h, with or without addition of 100 U/ml recombinant human IL-2. Cells were harvested for total RNA preparation and relative mRNA expression was determined by QPCR. (A) Bcl6 gene expression from C57BL/6 naïve CD4+ T cells cultured under complete or glucose deprivation medium with or without rhIL-2 addition for 72 h (n=3, mean ± SEM). (B) Bcl6 gene expression from IL-2 KO naïve CD4+ T cells cultured under complete or glucose deprivation medium with or without rhIL-2 addition for 72 h (n=3, mean ± SEM). NS (not significant, p> 0.05, two-way ANOVA), ***p < 0.001 (two-way ANOVA). Data are representative of 2 independent experiments of each time point with similar results.